Chimeric antigen receptors and methods of use thereof
AssigneeDana-Farber Cancer Institute, Inc.
InventorCarl Novina
Life of this patent
Estimating the term…
Abstract
The present invention provides chimeric antigen receptors, cells expressing same and methods of using same for treatment various disorders such as cancer, autoimmune disorders and graft vs host disease.
Claims25
A chimeric antigen receptor (CAR) for use in treating a subject in need thereof comprising an intracellular signaling domain, a transmembrane domain and an extracellular domain comprising i) the variable region of a T-cell receptor specific for a tumor-associated antigen or ii) the variable region of a T-cell receptor specific for a self-antigen, wherein said T-cell receptor is derived from a T-lymphocyte obtained from said subject.
(canceled)
(canceled)
(canceled)
A method of isolating a subject specific neoantigen comprising:
- a) isolating a plurality of class I and class H MHC peptides from a population of cells obtained from said subject;
- b) contacting the plurality of peptide isolated from step (a) with a T-cell receptor (TCR) multimer, wherein said TCR was isolated from a single T-cell isolated from said subject to form an TCR multimer-MHC peptide complex;
- c) isolating the MHC peptide from said complex.
-24. (canceled)
(canceled)
Dependencies are inferred from the claim text.