Enterobacter cloacae biocontrol strain capable of effectively inhibiting aspergillus flavus from synthesizing aflatoxins and application thereof
AssigneeOil Crops Research Institute, Chinese Acadamy of Agricultural Sciences
InventorsPeiwu LI, Xiaoxia DING, Qi ZHANG
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Abstract
The present invention belongs to the field of microorganisms, and particularly relates to an Enterobacter cloacae biocontrol strain capable of effectively inhibiting Aspergillus flavus from producing aflatoxins and an application thereof. Enterobacter cloacae biocontrol strain 3J1EC was deposited in China Center for Type Culture Collection on Jun. 13, 2017, with the deposit address being Wuhan University, Wuhan, China, and the deposit number being CCTCC No. M 2017330. The strain can be used for inhibiting Aspergillus flavus from producing aflatoxins to prevent and control the contamination of food crops by aflatoxins.
Claims6
An inhibitor for inhibiting production of aflatoxins by Aspergillus flavus, comprising a fermentation product of an Enterobacter cloacae biocontrol strain 3J1EC, and the Enterobacter cloacae biocontrol strain 3J1EC is deposited in China Center for Type Culture Collection (CCTCC) on Jun. 13, 2017, with an accession number being CCTCC No. M 2017330.
A method for preparing the fermentation broth of Enterobacter cloacae 3J1EC comprising:
- activating Enterobacter cloacae 3J1EC on an LB plate,
- culturing the Enterobacter cloacae in an incubator at 25° C.−37° C. for 24 h,
- picking up a single colony of Enterobacter cloacae with a needle,
- transferring the single colony to a liquid medium,
- culturing with shaking for 12 h-24 h, pipetting and transferring 1%-3% of culture liquid to a fresh liquid medium, and
- culturing with shaking for 12 h-24 h to obtain an antagonistic fermentation broth of strain Enterobacter cloacae 3J1EC,
- wherein the Enterobacter cloacae biocontrol strain 3J1EC is deposited in China Center for Type Culture Collection (CCTCC) with an accession number being CCTCC No. M 2017330
Dependencies are inferred from the claim text.