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US 2019/0159417 A1Published application
Method of generating polyploid plants
AssigneeUniversity of Bremen
InventorRita GROß-HARDT
Life of this patent
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Abstract
Polyploid plants obtainable by crossing of more than two parent plants within one generation as well as a novel high-throughput polypaternal breeding design (HIPOD) method allowing such polypaternal crossing are provided. In particular, triparental plants are described.
Claims20
A polyploid plant obtainable by crossing of one female parent plant with at least two male parent plants.
A transgenic tri-parental plant and/or progeny of such a plant, which comprises
- (i) a stably integrated first expression cassette comprising a nucleotide sequence encoding a promoter, preferably a constitutive promoter operably linked to a nucleotide sequence encoding a transactivator and a second expression cassette comprising a target nucleotide sequence, capable of being activated by said transactivator polypeptide operably linked to a nucleotide sequence which encodes a selectable marker, or
- (ii) a stably integrated first expression cassette comprising a nucleotide sequence encoding a promoter, preferably a constitutive promoter operably linked to a nucleotide sequence encoding a first selectable marker and a second expression cassette comprising a nucleotide sequence encoding a promoter, preferably a constitutive promoter, operably linked to a nucleotide sequence encoding a second selectable marker, wherein the selectable marker provides antibiotic, herbicide and/or antimetabolite resistance when expressed.
Dependencies are inferred from the claim text.