Models for analyzing data from sequencing-by-synthesis operations
AssigneeLife Technologies Corporation
InventorsTodd REARICK, Jonathan SCHULTZ
Life of this patent
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Abstract
Mathematical models for the analysis of signal data generated by sequencing of a polynucleotide strand using a pH-based method of detecting nucleotide incorporation(s). In an embodiment, the measured output signal from the reaction confinement region of a reactor array is mathematically modeled. The output signal may be modeled as a linear combination of one or more signal components, including a background signal component. This model is solved to determine the nucleotide incorporation signal. In another embodiment, the incorporation signal from the reaction confinement region of a reactor array is mathematically modeled.
Claims1
A method of sequencing a polynucleotide strand, comprising:
- flowing a series of nucleotide flows onto a reactor array having multiple reaction confinement regions, one or more copies of the polynucleotide strand being located in a loaded reaction confinement region of the reactor array;
- receiving output signals from the reactor array;
- determining a background signal for the loaded reaction confinement region using a model of an output signal from the loaded reaction confinement region;
- determining an incorporation signal from the loaded reaction confinement region using the model of an output signal from the loaded reaction confinement region;
- and
- determining an estimate of a number of nucleotides incorporated into the polynucleotide strand.
Dependencies are inferred from the claim text.