Microfabricated device with micro-environment sensors for assaying coagulation in fluid samples

A chip comprising:

• a micro-environment prothrombin time (PT) sensor;
• a micro-environment activated clotting time (ACT) sensor;
• and
• a counter reference electrode,
• wherein the micro-environment PT sensor and the micro-environment ACT sensor each comprise a polymer layer and diagnostic reagents configured in such a way that any reaction occurring in an immediate vicinity of each of the micro-environment sensors in a manner sufficient to achieve a signal at each of the micro-environment sensors will not detectably interfere or impact another reaction occurring at an adjacent sensor during normal usage,
• wherein the micro-environment PT sensor comprises PT diagnostic reagents, comprising at least one reagent for inducing extrinsic coagulation pathway in the biological sample, and
• wherein the micro-environment activated ACT sensor comprises ACT diagnostic reagents, comprising at least one reagent for inducing intrinsic coagulation pathway in the biological sample.

An integrated circuit chip comprising:

• a micro-environment prothrombin time (PT) sensor;
• a micro-environment activated partial thromboplastin time (aPTT) sensor or a micro-environment activated clotting time (ACT) sensor, wherein the micro-environment PT sensor is on a same vertical plane as the micro-environment aPTT sensor or the micro-environment ACT sensor;
• a first temporary electrical connector connected via a wiring to the micro-environment PT sensor;
• and
• a second temporary electrical connector connected via another wiring to the micro-environment aPTT sensor or the micro-environment ACT sensor.