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US 2019/0154700 A1Published application
C peptide detection by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsNigel CLARKE, Zhaohui CHEN
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Abstract
Methods are described for measuring the amount of C peptide in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying C peptide in a sample utilizing on-line extraction methods coupled with tandem mass spectrometric or high resolution/high accuracy mass spectrometric techniques.
Claims19
A method for determining the amount of C peptide in a sample by tandem mass spectrometry, the method comprising:
- (a) subjecting a sample suspected of containing C peptide to high performance liquid chromatography (HPLC) to obtain a fraction enriched in C peptide;
- (b) subjecting the enriched C peptide to an ionization source under conditions suitable to generate one or more C peptide ions detectable by mass spectrometry;
- (c) determining the amount of one or more C peptide ions by tandem mass spectrometry, wherein said determined ions comprise one or more fragment ions selected from the group of ions with mass to charge ratios consisting of 785.4±0.5 and 646.1±0.5;
- wherein the amount of ions determined in step (c) is related to the amount of a C peptide in said sample.
1 more element
A method for determining the amount of C peptide in a sample by tandem mass spectrometry, the method comprising:
- (a) subjecting a sample suspected of containing C peptide to solid phase extraction (SPE) and 1-D high performance liquid chromatography (1-D HPLC) to obtain a fraction enriched in C peptide;
- (b) subjecting the fraction enriched C peptide to an ionization source under conditions suitable to generate one or more C peptide ions detectable by mass spectrometry;
- (c) determining the amount of one or more C peptide ions by tandem mass spectrometry, wherein said determined ions comprise one or more fragment ions selected from the group of ions with mass to charge ratios consisting of 785.4±0.5 and 646.1±0.5;
- wherein the amount of ions determined in step (c) is related to the amount of a C peptide in said sample.
1 more element
Dependencies are inferred from the claim text.