Polymer-Based Lipid Nanodiscs And Macrodiscs

A method of making a lipid nanodisc, the method comprising:

• contacting:
• a lipid and
• a styrene/modified maleic anhydride copolymer comprising a pendant hydrophilic group and having a styrene to modified maleic anhydride molar ratio of about 1.1:1 to 1.5:1,
• to form a lipid nanodisc comprising a lipid bilayer comprising two opposing hydrophilic faces and a hydrophobic edge between the hydrophilic faces and the copolymer encircling the hydrophobic edge of the lipid bilayer.

A lipid nanodisc comprising:

• a lipid bilayer comprising two opposing hydrophilic faces and a hydrophobic edge between the hydrophilic faces;
• and
• a copolymer encircling the hydrophobic edge of the lipid bilayer, the copolymer comprising
• a first monomeric unit comprising a pendant aromatic group, and
• a second monomeric unit comprising a pendant hydrophilic group,
• wherein the first monomeric unit and the second monomeric unit are present in the copolymer in a molar ratio ranging from 1:1 to 3:1 for the first monomeric unit:the second monomeric unit.

A method of making a lipid nanodisc, the method comprising:

• contacting:
• a lipid;
• and
• a copolymer comprising
• a first monomeric unit comprising a pendant aromatic group, and
• a second monomeric unit comprising a pendant hydrophilic group,
• wherein the first monomeric unit and the second monomeric unit are present in the copolymer is a molar ratio ranging from 1:1 to 3:1 for the first monomeric unit:the second monomeric unit,
• to form a lipid nanodisc comprising a lipid bilayer comprising two opposing hydrophilic faces and a hydrophobic edge between the hydrophilic faces and the copolymer encircling the hydrophobic edge of the lipid bilayer.