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US 2019/0154697 A1Published application
Method for preparing and analyzing fluorescent compounds in plasma
AssigneeMediBeacon Inc.
InventorJeng J. Shieh
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Abstract
Disclosed herein is a method for analyzing the concentration of a fluorescent compound in the plasma of a patient. The method includes collecting a sample of plasma from a patient, diluting the sample with a solvent and analyzing the diluted sample by HPLC. The sample does not need to be dried down during sample preparation nor is an internal standard required.
Claims20
A method for measuring the amount of a fluorescent compound in plasma, the method comprising:
- collecting a sample of plasma,
- diluting the sample of plasma with at least one solvent;
- and
- analyzing the diluted sample by HPLC thereby measuring the amount of the compound in the plasma;
- wherein the sample of plasma is not dried before HPLC analysis, and no internal standard is added to the sample.
2 more elements
A method for measuring the amount of a fluorescent compound in plasma, the method comprising:
- collecting a sample of plasma,
- adding to the sample at least one solvent thereby causing plasma proteins to precipitate,
- removing the precipitated plasma proteins from the sample, and
- analyzing the sample by HPLC thereby measuring the amount of the compound in the plasma;
- wherein the sample is not dried before HPLC analysis, and no internal standard is added to the sample.
2 more elements
Dependencies are inferred from the claim text.