Method for detecting and isolating invasive cancer stem cells employing cell-surface amfr and the use thereof
AssigneesKun-Chih TSAI, Patrick Yuhmin YANG
InventorsKun-Chih TSAI, Patrick Yuhmin YANG, Tze-Sian CHAN
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Abstract
The present disclosure provides an isolated invasive cancer stem cell (iCSC) or a substantially homogeneous iCSC population comprising said iCSC, which is positive for the marker AMFR on the cell membrane. The present disclosure also provides a method of detecting the iCSC within an established cancer or a collection of cancer cells. The present disclosure also provides a method of isolating the iCSC or a substantially homogeneous cell population comprising said iCSC from an established cancer or a collection of cancer cells.
Claims13
-26. (canceled)
An isolated invasive cancer stem cell (iCSC) or a substantially homogeneous iCSC population comprising said iCSC, which is positive for the marker AMFR on the cell membrane.
A method of detecting an isolated invasive cancer stem cell (iCSC) which is positive for the marker AMFR on the cell membrane within an established cancer or a collection of cancer cells, wherein the method comprises:
- (a) preparing a sample of said cancer or said collection of cancer cells;
- (b) contacting said sample with an agent that binds to AMFR on the membrane;
- and (c) determining whether said sample contains cancer cells expressing AMFR on the cell membrane, regardless of the expression of AMFR in the cell cytosol or nuclei, thereby detecting said iCSC.
A method of isolating an isolated invasive cancer stem cell (iCSC) which is positive for the marker AMFR on the cell membrane or a substantially homogeneous cell population comprising said iCSC from an established cancer or a collection of cancer cells, wherein the method comprises:
- (a) preparing a sample of said cancer or said collection of cancer cells;
- (b) contacting said sample with a binding agent that binds to AMFR on the cell surface;
- and (c) isolating cancer cells from said sample that express AMFR on the cell membrane, thereby isolating said iCSC.
Dependencies are inferred from the claim text.