Poc test system and method

A test cassette for the detection of at least one analyte from a sample fluid, the test cassette having a first sample chamber and a second sample chamber, which are separated by a carrier having at least two surfaces comprising a front side and a rear side, wherein the front side of the carrier points towards the first sample chamber and the rear side of the carrier points towards the second sample chamber, and

• a) at least one receptor molecule fixed on the front side of the carrier,
• b) at least one free volume formed over each of the front side and the rear side of the carrier and delimiting the free volume in each case by a chamber wall,
• c) the first sample chamber having at least a first opening and a second opening, whereby the sample fluid flows through the first opening along a flow gradient over the front side of the carrier to the second opening distanced from the carrier,
• d) the second sample chamber having at least a first opening and a second opening, whereby the sample fluid flows through the first opening from the first sample chamber along a flow gradient over the rear side of the carrier,
• e) the second opening of the first sample chamber and the first opening of the second sample chamber being interconnected by a channel, the second opening of the second sample chamber enabling the discharge of the fluid,
• g) the free volumes from b.) being formed at least partially with a fluid column;
• and
• h) the flow gradient flowing along the front side and the rear side of the carrier by means of pressure application and entraining the fluid column from g.).