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US 2019/0154667 A1Published application
Organotypic pig retina culture system
OwnerAnnahita KERAVALA, Diana CEPEDAinventors
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Abstract
The present invention provides organotypic pig and non-human primate retina culture systems, and methods for evaluating or characterizing candidate therapeutic agents e.g. AAV-mediated gene vectors, using the same.
Claims73
An organotypic culture system comprising a retinal explant comprising live cells and a cell culture medium;
- wherein the retinal explant is obtained from an eye from a pig or a non-human primate.
A method of determining expression of an exogenous polynucleotide in a retinal explant, comprising:
- (A) isolating the retinal explant from an eye of a pig or a non-human primate, wherein the portion of the retinal explant comprises live cells;
- (B) contacting the retinal explant with the exogenous polynucleotide;
- (C) incubating the retinal explant in an organotypic culture system for a period of time;
- and
- (D) determining the expression of the exogenous polynucleotide in the retinal explant;
- thereby determining the expression of the exogenous polynucleotide;
- wherein the retinal explant is a porcine retinal explant or a non-human primate retinal explant.
4 more elements
Dependencies are inferred from the claim text.