Fluorescence histo-tomography (fht) systems and methods

A method of performing fluorescence histo-tomography (FHT) comprising:

• capturing, by an imaging device mounted within a chamber of a slicing apparatus, a white light image of a block face of a tissue specimen retained within the chamber;
• capturing, by the imaging device, a fluorescence image of the block face under white light and fluorescence illumination;
• co-registering, by the imaging device, the white light and fluorescence images to form a combined image;
• and
• providing, by the imaging device, the combined image to an electronic display.

A fluorescence histo-tomography (FHT) system comprising:

• a portable imaging housing having a handle to allow a user to position the imgaging housing within a chamber of a slicing apparatus;
• a camera located within the imaging housing, the camera configured to acquire both fluorescence and white light images;
• a fluorescence light source located within the imaging housing;
• and
• a support mount configured to support the imaging housing within the chamber such that the cameras and fluorescence light source are aimed towards a block face of a tissue specimen retained within the chamber.