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US 2019/0153552 A1Published application
Hpv screening platform
AssigneeMayo Foundation for Medical Education and Research
InventorsMarina R. Walther-Antonio, Yuguang Liu
Life of this patent
Estimating the term…
Abstract
Materials and methods are provided herein for determining if a subject has, or is at risk of developing, a clinical condition (e.g., cervical cancer). For example, this document provides a cost-effective self-test for determining whether a biological fluid from a subject contains a high-risk HPV strain, and a method for use of the self-test.
Claims21
A kit comprising:
- (a) a receptacle for receiving a biological fluid sample, the receptacle having a first nucleic acid reversibly attached to a first interior surface and a second nucleic acid immobilized on a second interior surface, wherein the first nucleic acid is complementary to a first nucleic acid sequence of a selected marker, and wherein the second nucleic acid is complementary to a second nucleic acid sequence of the marker;
- and
- (b) a substrate comprising a first portion with an integrated positive control result and a second portion for receiving a test fluid.
10-18. (canceled)
A method for determining that a biological fluid contains a selected marker, comprising:
- (a) providing a receptacle for receiving the biological fluid, the receptacle having a first nucleic acid reversibly attached to a first interior surface and a second nucleic acid immobilized on a second interior surface, wherein the first nucleic acid is complementary to a nucleic acid sequence of the marker and is labeled with a means for visual detection, and wherein the second nucleic acid is complementary to a second nucleic acid sequence of the marker;
- (b) placing a sample of the biological fluid into the receptacle, such that the first nucleic acid is released from the first interior surface, and the first and second nucleic acids hybridize to the first and second complementary nucleic acid sequences of the marker, such that the first nucleic acid becomes attached to the second interior surface via the marker and the second nucleic acid;
- (c) removing the biological fluid from the receptacle;
- (d) washing the interior of the receptacle to remove residual biological fluid;
- (e) placing a substrate that interacts with the means for visual detection into the receptacle, such that the means for visual detection can act on the substrate to generate a signal;
- and
- (f) visually inspecting the vial containing the substrate, or a sample of the substrate, to determine that the signal is present, thus indicating the presence of the high-risk HPV strain in the biological fluid.
4 more elements
29-38. (canceled)
Dependencies are inferred from the claim text.