Methods for gynecologic neoplasm diagnosis

A method for determining the methylation state of a target gene as a biomarker in a sample from a subject to assess whether the subject has an endometrial carcinoma, comprising the steps of:

• (a) obtaining a sample from the subject;
• (b) determining a methylation state of at least one target gene in the sample, wherein the at least one target gene comprises at least one of BHLHE22, CELF4, CLVS2 and THY1;
• (c) determining whether the at least one target gene is hypermethylated;
• and (d) assessing whether the subject has the endometrial carcinoma based on the result of the step (c), wherein the hypermethylation of the at least one target gene indicates that the subject has the endometrial carcinoma.

A method for assessing whether a subject has a ovarian carcinoma or colon carcinoma, comprising the steps of:

• (a) obtaining a sample from the subject;
• (b) determining the methylation state of at least one target gene in the sample, wherein the at least one target gene is selected from the group consisting of, BHLHE2, CELF4 and CLVS2;
• (c) determining whether the at least one target gene is hypermethylated;
• and (d) assessing whether the subject has the ovarian carcinoma or colon carcinoma based on the result of the step (c), wherein the hypermethylation of the at least one target gene indicates that the subject has the ovarian carcinoma or colon carcinoma.