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US 2019/0153509 A1Published application
Molecular tagging methods and sequencing libraries
AssigneeThe Translational Genomics Research Institute
InventorsTimothy K. McDaniel, Muhammed Murtaza
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Abstract
The invention provides a method for validating patient-specific oligos using spike-in sequences.
Claims20
A method of validating a patient-specific oligo, the method comprising:
- extracting DNA or RNA from a patient sample to produce a DNA or RNA sample;
- introducing a plurality of sets of spike-in sequences into the DNA or RNA sample to produce a spiked sample, wherein the plurality of sets of spike-in sequences comprises spike-in sequences of known concentrations;
- cosynthesizing synthesis-control oligos and the patient-specific oligo;
- and
- assaying the spiked sample with the synthesis-control oligos to produce a read out of the counts of the set of spike-in sequences;
- and
- comparing the counts from the set of spike-in sequences with the known concentrations of the spike-in sequences, wherein the limit of detection (LOD) and the dynamic range for the patient-specific oligo are validated when the counts from the set of spike-in sequences correspond to the known concentrations of the spike-in sequences.
4 more elements
A method of validating a patient-specific oligo, the method comprising:
- extracting DNA or RNA from a patient sample to produce a DNA or RNA sample;
- introducing a plurality of sets of spike-in sequences into the DNA or RNA sample to produce a spiked sample, wherein the plurality of sets of spike-in sequences comprises spike-in sequences of known concentrations;
- assaying the spiked sample with synthesis-control oligos to produce a read out of the counts of the set of spike-in sequences, wherein the synthesis-control oligos are cosynthesized with the patient-specific oligo;
- and
- comparing the counts from the set of spike-in sequences with the known concentrations of the spike-in sequences, wherein the LOD and the dynamic range for the patient-specific oligo are validated when the counts from the set of spike-in sequences correspond to the known concentrations of the spike-in sequences.
2 more elements
Dependencies are inferred from the claim text.