Method for inline bilayer capacitance monitoring
AssigneeRoche Sequencing Solutions, Inc.
InventorAshraf Wahba
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Abstract
A method of detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip is disclosed. A lipid membrane is coupled with an integrating capacitor, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage is determined. A state of the lipid membrane is determined based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage.
Claims20
A method of detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip, comprising:
- applying an alternating current (AC) voltage across a lipid membrane disposed between a working electrode and a counter electrode, the lipid membrane electrically coupled with an integrating capacitor;
- periodically sampling a voltage across the integrating capacitor;
- determining a change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage;
- and
- detecting a state of the lipid membrane based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage.
A system for detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip, comprising:
- an integrating capacitor;
- a working electrode electrically coupled to the integrating capacitor;
- a counter electrode;
- a surface configured to support a lipid membrane between the working electrode and the counter electrode such that the lipid membrane is electrically coupled with the integrating capacitor;
- an alternating current (AC) voltage source configured to apply an AC voltage to the counter electrode;
- an analog measurement circuitry configured to periodically sample a voltage across the integrating capacitor;
- and
- a processor or a circuitry configured to:
- determine a change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage;
- and
- detect a state of the lipid membrane based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage.
Dependencies are inferred from the claim text.