Assay for the comprehensive identification of antibiotic sensitivity
AssigneeCAP Diagnostics, LLC, dba Pathnostics
InventorsDavid A. Baunoch, Miguel F.R. Penaranda, Michael L. Opel, Maher Badir
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Abstract
Disclosed herein are methods determining susceptibility of bacteria in a sample from a subject suspected of having an infection to a plurality of antibiotics simultaneously, wherein the sample is tested without first isolating the bacteria from the sample.
Claims22
A method for simultaneously determining susceptibility of a mixed population of bacteria in a sample to a plurality of antibiotics, the method comprising:
- applying a quantity of the sample simultaneously to a plurality of antibiotic-containing medium, wherein the sample is from a subject suspected of having an infection;
- culturing the sample on the medium for a period of time;
- suspending bacteria present in the medium with a solution and recovering the suspension;
- measuring an OD600 of the suspension, wherein an adjusted OD600 of a suspension indicates the susceptibility or resistance of bacteria in the sample to an individual antibiotic;
- and
- providing the susceptibility information to a medical professional to determine antibiotic therapy for the subject.
A method for simultaneously determining susceptibility of a mixed population of bacteria in a sample to a plurality of antibiotics, the method comprising:
- mixing a quantity of a sample with growth medium and incubating for a period of time;
- applying the sample simultaneously to a plurality of antibiotic-containing medium, wherein the sample is from a subject suspected of having an infection;
- culturing the sample in the antibiotic-containing medium for a period of time;
- measuring bacterial growth in the medium by OD600 measurement, wherein an adjusted OD600 measurement of the sample indicates the susceptibility or resistance of the bacteria in the sample to an individual antibiotic;
- and
- providing the susceptibility information to a medical professional to determine antibiotic therapy for the subject.
Dependencies are inferred from the claim text.