Methods for modulating protein galactosylation profiles of recombinant proteins using peracetyl galactose
AssigneeAres Trading S.A.
InventorsMARTIN JORDAN, GABRIELLE LECLERCQ, JONATHAN SOUQUET, HERVE BROLY, DAVID BRUHLMANN
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Abstract
The present invention relates to methods and compositions for modulating glycosylation profile, such as the galactosylation profile, of recombinant proteins expressed by mammalian host cells during the cell culture process by supplementing cell culture media with a peracetyl galactose.
Claims13
1-15. (canceled)
A method of producing a recombinant protein with a modulated galactosylation profile, said method comprising culturing a host cell expressing said protein in:
- a) cell culture medium comprising a peracetyl galactose;
- or b) cell culture medium complemented with at least one feed comprising a peracetyl galactose.
A composition comprising a cell culture medium comprising a peracetyl galactose or supplemented with a peracetyl galactose.
Dependencies are inferred from the claim text.