Recombinant polynucleotide involved in lactone synthesis and process for synthesis of lactones thereof
AssigneeCouncil of Scientific and Industrial Research
InventorsVidya Shrikant Gupta, Ashish Balwant Deshpande, Pranjali Sudhir Oak, Ashok Prabhakar Giri
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Abstract
The present invention provides the polynucleotide encoding enzyme involved in lactone synthesis and a process for synthesis of lactones using said polynucleotides. The invention also provides recombinant plasmid expression vector comprising said polynucleotide sequence. The recombinant protein encoded by said polynucleotides leads to synthesis of lactones having flavour peculiar to Alphonso mangoes.
Claims10
A recombinant polynucleotide involved in lactone synthesis, wherein the polynucleotide is selected from the group consisting of:
- (a) a polynucleotide sequence as set forth in SEQ ID No. 1 encoding a recombinant polypeptide epoxide hydrolase 2 of SEQ ID No. 3;
- and
- (b) a polynucleotide sequence as set forth in SEQ ID No. 4 encoding a recombinant polypeptide peroxygenase of SEQ ID No. 6.
A process for synthesis of lactones, said process comprising:
- (a) synthesizing polynucleotide sequence as set forth in SEQ ID No.:
- 4;
- (b) expressing a recombinant construct carrying said polynucleotide sequence of SEQ ID No. 4 in a host cell to obtain recombinant polypeptide peroxygenase of SEQ ID No. 6 wherein the host cell is selected from the group consisting of E. coli BL21 or E. coli Rosetta;
- (c) catalyzing conversion of unsaturated fatty acids to epoxy fatty acids in presence of the recombinant polypeptide peroxygenase of SEQ ID No. 6 of step (b) to obtain epoxy fatty acids;
- (d) synthesizing polynucleotide sequence as set forth in SEQ ID No.:
- 1;
- (e) expressing a recombinant construct carrying said polynucleotide sequence of SEQ ID No. 1 in a host cell to obtain recombinant polypeptide peroxygenase of SEQ ID No. 3;
- (f) catalyzing conversion of the epoxy fatty acids from step (c) to di-hydroxy fatty acids in presence of the recombinant polypeptide peroxygenase of SEQ ID No. 3 of step (e) to obtain di-hydroxy fatty acids;
- and
- (g) subjecting the di-hydroxy fatty acids of step (f) to multiple cycles of to and β oxidation to obtain the lactones.
Dependencies are inferred from the claim text.