Transformant and process for production thereof, and process for production of lactic acid
AssigneeJMTC Enzyme Corporation
InventorsFutoshi HARA, Shuichiro KIMURA, Yuichiro HAGIYA, Takayuki TANAKA
Life of this patent
Estimating the term…
Abstract
The present invention relates to a transformant which uses Schizosaccharomyces pombe as a host into which a D-LDH gene derived from bacteria of the genus Pediococcus and a D-LDH gene derived from bacteria of the genus Lactobacillus are incorporated and in which some of the genes in a group of pyruvate decarboxylase-encoding genes of the Schizosaccharomyces pombe host have been deleted or inactivated.
Claims13
A transformant which uses Schizosaccharomyces pombe as a host into which a D-lactate dehydrogenase gene derived from bacteria of the genus Pediococcus and a D-lactate dehydrogenase gene derived from bacteria of the genus Lactobacillus are incorporated,
- wherein some of the genes in a group of pyruvate decarboxylase-encoding genes of the Schizosaccharomyces pombe host have been deleted or inactivated.
A process for production of a transformant using Schizosaccharomyces pombe as a host into which a D-lactate dehydrogenase gene derived from bacteria of the genus Pediococcus and a D-lactate dehydrogenase gene derived from bacteria of the genus Lactobacillus are incorporated and in which some of the genes in a group of pyruvate decarboxylase-encoding genes of the Schizosaccharomyces pombe host have been deleted or inactivated, the process comprising:
- a step of obtaining a transformant by introducing an expression cassette into the host,
- wherein the expression cassette consists of an expression cassette including a promoter and a terminator functioning in the Schizosaccharomyces pombe and a D-lactate dehydrogenase gene derived from bacteria of the genus Pediococcus and an expression cassette including a promoter and a terminator functioning in the Schizosaccharomyces pombe and a D-lactate dehydrogenase gene derived from bacteria of the genus Lactobacillus, or consists of an expression cassette including a promoter or a terminator functioning in the Schizosaccharomyces pombe, a D-lactate dehydrogenase gene derived from bacteria of the genus Pediococcus, and a D-lactate dehydrogenase gene derived from bacteria of the genus Lactobacillus, and
- a host in which some of the genes in a group of pyruvate decarboxylase-encoding genes have been deleted or inactivated is used as the host, or
- some of the genes in a group of pyruvate decarboxylase-encoding genes of the obtained transformant are deleted or inactivated.
Dependencies are inferred from the claim text.