Mutant alkb gene
AssigneeEvonik Degussa GmbH
InventorsArne SKERRA, Ludwig KIRMAIR, Steffen SCHAFFER, Christoph SCHORSCH, Mirja WESSEL, Thomas HAAS
Life of this patent
Estimating the term…
Abstract
There is provided a microbial cell expressing a mutant AlkB enzyme, the mutant AlkB enzyme comprising at least one point mutation in the wild type sequence of AlkB, wherein the point mutation is at amino acid position V129 and/or T136 of the wild type AlkB enzyme. There is also provided a method for producing omega-hydroxy carboxylic acid and/or ester thereof using this cell.
Claims15
A microbial cell expressing a mutant AlkB enzyme, the mutant AlkB enzyme comprising at least one point mutation in the wild type sequence of AlkB, wherein the point mutation is at amino acid position V129 and/or T136 of the wild type AlkB enzyme wherein the point mutation at amino acid V129 is selected from the group consisting of V129T and V129A.
A method of producing at least one omega-hydroxy carboxylic acid and/or ester thereof from at least one carboxylic acid and/or ester thereof as substrate, the method comprising
- contacting at least one genetically modified cell expressing a mutant AlkB enzyme with the substrate carboxylic acid and/or ester thereof,
- wherein the substrate carboxylic acid and/or ester thereof is a C6-C14 carboxylic acid and/or ester thereof;
- and
- the mutant AlkB enzyme comprises at least one point mutation in the wild type sequence of AlkB, wherein the point mutation is at amino acid position selected from the group consisting of V129, L132 and T136 of the wild type AlkB enzyme.
Dependencies are inferred from the claim text.