Novel glucose dehydrogenase
AssigneeAmano Enzyme Inc.
InventorsKyoichi Nishio, Yuzo Kojima, Shotaro Yamaguchi
Life of this patent
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Abstract
Intended is to provide a highly practical novel FAD-GDH. A glucose dehydrogenase having the following properties is provided: (1) action: catalyzes the reaction of oxidizing hydroxyl groups of glucose to form glucono-δ-lactone in the presence of an electron acceptor; (2) substrate specificity: reactivity to D-xylose is 10% or less when the reactivity to D-glucose is 100%; (3) pH stability: stable at pH 5 to 8; (4) amino acid sequence: including the amino acid sequence set forth in SEQ ID NO: 1, or an amino acid sequence with an identity of 83% or more to the amino acid sequence.
Claims16
A glucose dehydrogenase comprising the following properties:
- (1) action:
- catalyzes the reaction of oxidizing hydroxyl groups of glucose to form glucono-δ-lactone in the presence of an electron acceptor;
- (2) substrate specificity:
- reactivity to D-xylose is 10% or less when the reactivity to D-glucose is 100%;
- (3) pH stability:
- stable at pH 5 to 8;
- and
- (4) amino acid sequence:
- comprising the amino acid sequence set forth in SEQ ID NO:
- 1, or an amino acid sequence with an identity of 83% or more to the amino acid sequence.
A glucose dehydrogenase comprising the following properties:
- (1) action:
- catalyzes the reaction of oxidizing hydroxyl groups of glucose to form glucono-δ-lactone in the presence of an electron acceptor;
- (2) substrate specificity:
- reactivity to D-xylose is 10% or less when the reactivity to D-glucose is 100%;
- (3) pH stability:
- stable at pH 5 to 8;
- and
- (4) molecular weight:
- about 60 kDa (as measured by SDS-PAGE).
A glucose dehydrogenase gene comprising any DNA selected from the group consisting of the following (A) to (C):
- (A) a DNA coding the amino acid sequence set forth in SEQ ID NO:
- 1;
- (B) a DNA composed of the base sequence set forth in SEQ ID NO:
- 2;
- and
- (C) a DNA having a base sequence equivalent to the base sequence set forth in SEQ ID NO:
- 2, and coding a protein having glucose dehydrogenase activity.
Dependencies are inferred from the claim text.