Method for preparing pluripotent stem cell-derived cardiomyocyte population

A method for preparing a cardiomyocyte population for transplantation therapy, the method comprising the steps of:

• (1) inducing pluripotent stem cells to differentiate into cardiomyocytes,
• (2) bringing a cell population obtained by the induced differentiation into contact with a laminin selected from the group consisting of laminin α2β1γ1, laminin α2β2γ1, laminin α1β1γ1 and laminin α1β2γ1, or a fragment thereof having integrin binding activity, and
• (3) retrieving cells adherent to the laminin or the laminin fragment,
• wherein the contact of the cell population with the laminin or the laminin fragment in step (2) is achieved by adding a cell suspension containing the cell population obtained in step (1) to a culture vessel having a culture surface coated with the laminin or the laminin fragment, followed by a certain period of incubation;
• by adding a carrier coated with the laminin or the laminin fragment to a cell suspension containing the cell population obtained in step (1), followed by a certain period of incubation;
• or by passing a cell suspension containing the cell population obtained in step (1) through a hollow fiber membrane having an inner surface coated with the laminin or the laminin fragment, and
• wherein step (3) comprises removing cells not adherent to the laminin or the laminin fragment and then detaching cells adherent to the laminin or the laminin fragment.

A method for increasing the purity of cardiomyocytes in a cell population obtained by induced differentiation of pluripotent stem cells into cardiomyocytes, the method comprising

• step 1:
• bringing the cell population into contact with a laminin selected from the group consisting of laminin α2β1γ1. laminin α2β2γ1, laminin α1β1γ1 and laminin α1β2γ1, or a fragment thereof having integrin binding activity, and
• step 2:
• retrieving cells adherent to the laminin or the laminin fragment,
• wherein the contact of the cell population with the laminin or the laminin fragment in step 1 is achieved by adding a cell suspension containing the cell population to a culture vessel having a culture surface coated with the laminin or the laminin fragment, followed by a certain period of incubation;
• by adding a carrier coated with the laminin or the laminin fragment to a cell suspension containing the cell population, followed by a certain period of incubation;
• or by passing a cell suspension containing the cell population through a hollow fiber membrane having an inner surface coated with the laminin or the laminin fragment, and
• wherein step 2 comprises removing cells not adherent to the laminin or the laminin fragment and then detaching cells adherent to the laminin or the laminin fragment.