Improved microbial production of fats
AssigneeWilliam Marsh Rice University
InventorsKa-Yiu SAN, Zhilin LI, Xian ZHANG
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Abstract
This invention describes a method of using microbial to produce fats, such as fatty acids and their derivatives, or products derived from the fatty acid synthesis cycle, such as hydroxyfatty acids, methyl ketones, and the like.
Claims29
A method of producing products in bacteria, comprising:
- a) aerobically culturing a bacteria in a growth medium until sufficient cell mass is reached;
- b) smoothly transitioning from aerobic culturing to culturing under oxygen lean conditions (>0.1% DO to <5% DO) over a course of time, preferably 1-12 hrs, 2-8 hrs, or about 5 hrs;
- c) further culturing said bacteria under oxygen lean conditions by only sparging the head space with O2 containing gas until product is formed;
- and
- d) isolating said product from said bacteria, said growth medium or both.
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A method of producing fatty acids in bacteria, said bacteria comprising an overexpressed NADH-dependent formate dehydrogenase, an overexpressed NADH insensitive pyruvate dehydrogenase or an overexpressed pyruvate:ferredoxin oxidoreductase;
- said method comprising:
- a) aerobically culturing a bacteria in a growth medium until >75 to <95% maximum cell mass before stationary phase is reached;
- b) simultaneously smoothly transitioning from aerobic to anaerobic or oxygen lean conditions of 0 to <5% DO over a course of time;
- c) further culturing said bacteria with anaerobic or oxygen lean conditions until fatty acids are formed;
- and
- d) isolating said fatty acids from said bacteria, said growth medium or both.
A recombinant bacteria comprising at least two chromosomally integrated copies of an exogenous NAD-dependent formate dehydrogenase (2× or 3×FDH).
Dependencies are inferred from the claim text.