Dna constructs for manufacturing bio-therapuetic polypeptides for use in animal vaccines and therapeutics
AssigneeMicroSynbiotiX, Ltd.
InventorsViktor Antonio MacKenzie-Lamb, Simon Porphy Jegathese, Kwang-Chul Kwon
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Abstract
A nucleic acid constructs including a heterologous nucleotide sequence operably linked to a constitutively active promoter; a 5′ recombination sequence at a 5′ end of the construct and a 3′ recombination sequence at a 3′ end of the construct, wherein the recombination sequences are configured for homologous recombination into a genome of a microalgal host cell; and optionally a bacterial 5′ untranslated region (5′UTR) between the 5′ recombination sequence and the promoter, and a bacterial 3′ untranslated region (3′UTR) between the 3′ recombination sequence and the heterologous nucleotide sequence.
Claims15
A nucleic acid construct comprising:
- a) a heterologous nucleotide sequence operably linked to a constitutively active promoter;
- and
- b) a 5′ recombination sequence at a 5′ end of the construct and a 3′ recombination sequence at a 3′ end of the construct, wherein the recombination sequences are configured for homologous recombination into a genome of a microalgal host cell.
A nucleic acid construct comprising:
- a) a multicloning site configured for cleavage by a plurality of different restriction endonucleases;
- b) a constitutively active promoter that directs transcription towards the multicloning site;
- c) a 5′ recombination sequence at a 5′ end of the construct and a 3′ recombination sequence at a 3′ end of the construct, wherein the recombination sequences are configured for homologous recombination into a genome of a microalgal host cell;
- and
- d) a bacterial 5′ untranslated region (5′UTR) between the 5′ recombination sequence and the promoter, and a bacterial 3′ untranslated region (3′UTR) between the 3′ recombination sequence and the multicloning site.
Dependencies are inferred from the claim text.