Optical sectioning apparatus using advanced mirau optical interference microscopy

An optical sectioning apparatus combining Mirau optical interference microscopy and fluorescence microscopy, including:

• a wide band light source apparatus for generating a wide band light beam;
• an optical circulator, having a first side, a second side, and a third side, the first side facing the wide band light source apparatus for splitting an incident light beam into a reflected light beam traveling out the second side and a transmitted light beam;
• a short wavelength light source apparatus for generating a short wavelength light beam;
• a first dichroic splitter, having a first side, a second side, and a third side, with the first side facing the short wavelength light source apparatus, the third side facing the second side of the optical circulator, the first dichroic splitter being capable of providing a light-blocking effect on a band of wavelengths shorter than a preset wavelength, and the short wavelength light beam having a wavelength shorter than the preset wavelength;
• a Mirau interference objective lens, having a collimated side and a focal side, the collimated side facing the second side of the first dichroic splitter;
• a sample carrier unit facing the focal side of the Mirau interference objective lens and being used for carrying a sample stained with a fluorescent dye;
• a projection lens, having a light entrance side and a light exit side, with the light entrance side facing the third side of the optical circulator;
• and
• a sensor unit facing the light exit side of the projection lens.