Nucleic acid probe with single fluorophore label bound to internal cytosine for use in loop mediated isothermal amplification
AssigneeMast Group Limited
InventorsMonika Iwona Suwara, Sajid Javed, Elizabeth Ann Gillies
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Abstract
The disclosure relates to novel probes for use in LAMP detection methods. The probes contain a single fluorophore label bound to an internal cytosine residue of the probe. The probes are particularly useful in the detection of chlamydia and gonorrhea infections in a patient.
Claims22
A probe for isothermal nucleic acid amplification comprising an oligonucleotide probe sequence complementary to a region of a target nucleic acid sequence, wherein said oligonucleotide probe sequence has only one fluorophore label and which label is bound to an internal cytosine base and wherein said oligonucleotide probe sequence does not have a 3′ end terminator.
Dependencies are inferred from the claim text.