Unsaturated amino acids

Abstract

There is provided a method of producing at least one unsaturated amino acid from at least one amino acid comprising at least two carbonyl groups, the method comprising (a) contacting a recombinant microbial cell with a medium comprising the amino acid comprising the carbonyl groups, wherein the cell is genetically modified to comprise—at least a first genetic mutation that increases the expression relative to the wild type cell of an enzyme (E) selected from the CYP152 10 peroxygenase family, and—at least a second genetic mutation that increases the expression relative to the wild type cell of at least one NAD(P)+ oxidoreductase (E2) and the corresponding mediator protein.

Dependencies are inferred from the claim text.