Messenger rna based viral production
AssigneeTranslate Bio, Inc.
InventorsMichael Heartlein, Frank DeRosa, Lianne Smith
Life of this patent
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Abstract
The present invention provides methods for producing recombinant viral particles based on the use of exogenous mRNAs to supply various helper factors for assembly of viral particles, purified recombinant viral particles produced using such methods, and methods of using such viral particles.
Claims29
A method of producing a lentiviral particle, the method comprising:
- introducing into a packaging cell one or more exogenous mRNAs encoding one or more helper factors for assembling transduction-competent lentiviral particles, wherein the packaging cell comprises a gene of interest;
- and
- culturing the packaging cell under conditions suitable for the packaging cell to produce a lentiviral particle comprising the gene of interest.
(canceled)
12-16. (canceled)
18-21. (canceled)
A method of producing a lentiviral particle, the method comprising
- introducing into a packaging cell (i) an exogenous mRNA encoding a lentiviral Gag protein, (ii) an exogenous mRNA encoding a lentiviral Pol protein, and (iii) an exogenous mRNA encoding a lentiviral Env protein;
- wherein the packaging cell comprises a gene of interest associated with a packaging signal;
- and
- culturing the packaging cell under conditions suitable for the packaging cell to produce a lentiviral particle comprising the gene of interest.
(canceled)
A packaging cell capable of producing a lentiviral particle, comprising:
- one or more exogenous mRNAs encoding one or more helper factors for assembling transduction-competent lentiviral particles, wherein the packaging cell comprises a gene of interest associated with a packaging signal.
27-35. (canceled)
A system for producing a lentiviral particle, comprising
- one or more constructs encoding one or more helper factors for assembling transduction-competent lentiviral particles;
- reagents for in vitro transcription of mRNAs from the one or more constructs encoding one or more helper factors;
- a packaging cell;
- and
- reagents for introducing the in vitro transcribed mRNAs into the packaging cell.
(canceled)
A method of producing an adeno-associated virus (AAV) particle, the method comprising:
- introducing into a packaging cell one or more exogenous mRNAs encoding one or more helper factors for assembling transduction-competent AAV particles, wherein the packaging cell comprises a gene of interest;
- and
- culturing the packaging cell under conditions suitable for the packaging cell to produce an AAV particle comprising the gene of interest.
39-52. (canceled)
54-77. (canceled)
80-89. (canceled)
Dependencies are inferred from the claim text.