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AssigneeThe Regents of the University of California
InventorsDino Di Carlo, Janay Kong, Aydogan Ozcan, Omai Garner
Estimating the term…
A fluorescent dye and quencher mixture for reporting on nucleic acid amplification from a sample includes a fluorescent intercalating dye, a dye sequestering or quenching agent such as hydroxynapthol blue (HNB) or caffeine, and primers, dNTPs, and a nucleic acid polymerizing enzyme or fragment thereof. The presence of the dye in combination with the dye sequestering or quenching agent improves the overall dynamic range of the fluorescent signal as well as shortens the time needed for visualization or image capture of amplified nucleic acid. The fluorescent dye and quencher mixture also enables the detection of nucleic acids in samples having low copy numbers.
A fluorescent dye and quencher mixture for reporting on nucleic acid amplification from a sample comprising:
(canceled)
A fluorescent dye and quencher mixture for reporting on nucleic acid amplification from a sample using loop-mediated isothermal amplification (LAMP) comprising:
A fluorescent dye and quencher mixture for reporting on nucleic acid amplification from a sample comprising:
(canceled)
A method of improving the fluorescent reporting of a nucleic amplification process that uses a fluorescent intercalating dye, the method comprising:
A fluorescent dye and quencher mixture for reporting on nucleic acid concentration from a sample comprising:
Dependencies are inferred from the claim text.