# US 2019/0119694 A1 · Enhanced cannabis plants and methods of making and using the same > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2019/0119694 A1 - Document ID: US20190119694A1 - Status: Published application - Kind: A1 - Application number: 16/093,066 - Filed: 2017-04-14 - Published: 2019-04-25 - Earliest claimed: 2016-04-14 - Assignees: Ebbu, LLC - Applicants: ebbu, INC. - Inventors: Robert Fletcher ROSCOW, Jr. - Web page: https://patentgrep.com/patent/US20190119694A1 ## CPC - C12N 15/8243: {involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine} - A01H 6/28: Cannabaceae, e.g. cannabis - A61K 31/658: {o-phenolic cannabinoids, e.g. cannabidiol, cannabigerolic acid, cannabichromene or tetrahydrocannabinol} - C07K 14/415: from plants - C12N 15/82: for plant cells {, e.g. plant artificial chromosomes (PACs)} - C12N 15/8218: {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]} - C12N 15/8261: {with agronomic (input) traits, e.g. crop yield} - Y02A 40/146: Genetically Modified [GMO] plants, e.g. transgenic plants - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D) - C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00) - Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE ## Abstract Disclosed herein is a plant of the genus cannabis that does not require flowering in order to produce trichomes comprising secondary compounds. Provided herein is a plant of the genus cannabis that has trichomes on non-flowering parts of the plant, such as leaves. The disclosed plants have a high mass % of secondary compounds and a high degree of trichome coverage on the surface of the plant. Also disclosed herein are methods of producing secondary compounds from a plant of genus cannabis without flowering the plant of genus cannabis. For example, the disclosed methods provide for inducing trichome development on a plant of genus cannabis without flowering the plant of genus cannabis. ## Claims ### Claim 1 A plant of genus cannabis, having a surface area and comprising trichomes on non-flowering parts of the plant. ### Claim 2 (depends on 1) The plant of claim 1 comprising trichomes on 25-100% of the surface area of plant. ### Claim 3 (depends on 2) The plant of claim 2, comprising trichomes on 50-100% of the surface area of plant. ### Claim 4 (depends on 2) The plant of claim 2, comprising trichomes on 70-100% of the surface area of plant. ### Claim 5 (depends on 1) The plant of claim 1, comprising 20-80 mass % secondary compounds. ### Claim 6 (depends on 5) The plant of claim 5, comprising 30-70 mass % secondary compounds. ### Claim 7 (depends on 5) The plant of claim 5, comprising 40-65 mass % secondary compounds. ### Claim 8 (depends on 5) The plant of claim 5, comprising 30-60 mass % secondary compounds. ### Claim 9 A plant of the genus cannabis comprising cDNA fragments corresponding to trichome induction in a plant not of genus cannabis. ### Claim 10 (depends on 9) The plant of claim 9, wherein the plant not of the genus cannabis is a plant of genus Arabidopsis. ### Claim 11 A method of producing secondary compounds comprising inducing trichome development in a plant of the genus cannabis. ### Claim 12 (depends on 11) The method of claim 11, wherein said secondary compounds are chosen from cannabinoids or terpenes. ### Claim 13 (depends on 11) The method of claim 11, comprising harvesting at least one cannabinoid or at least one terpene during the vegetative growth cycle of the plant of the genus cannabis. ### Claim 14 (depends on 11) The method of claim 11, comprising modifying genetic material of the plant of the genus cannabis. ### Claim 15 (depends on 11) The method of claim 11, comprising introducing non-native DNA to the plant of the genus cannabis. ### Claim 16 (depends on 11) The method of claim 11, comprising introducing additional copies DNA native to the plant of the genus cannabis. ### Claim 17 (depends on 14) The method of claim 14, comprising overexpressing at least one trichome induction gene. ### Claim 18 (depends on 17) The method of claim 17, wherein the at least one trichome induction gene is chosen from the bHLH, WD40 repeat protein, R2R3-MYB, or R3-MYB families. ### Claim 19 (depends on 14) The method of claim 14, comprising infecting cells of the plant of genus cannabis with a transformed bacterium. ### Claim 20 (depends on 19) The method of claim 19, comprising infecting cells of the plant of genus cannabis with a transformed bacterium via syringe infiltration. ### Claim 21 (depends on 19) The method of claim 19, comprising infecting cells of the plant of genus cannabis with a transformed bacterium via vacuum infiltration. ### Claim 22 (depends on 14) The method of claim 14, comprising treating cells of the plant of genus cannabis with DNA attached to a metal particle via biolistic particle delivery. ### Claim 23 (depends on 14) The method of claim 14, comprising: repressing post-transcriptional processing of a transcriptional repressor of trichome induction; and repressing expression of a target gene as functional protein. ### Claim 24 (depends on 23) The method of claim 23, comprising: expressing an RNA interference molecule corresponding to a trichome induction repression gene; and reducing translation of mRNA trichome induction repression genes into functional protein. ### Claim 25 (depends on 23) The method of claim 23, comprising modifying expression of GLABROUS INFLORESENCE STEMS (GIS); GLABROUS INFLORESENCE STEMS 2 (G152); GLABORUS INFLORESCENCE STEMS 3 (GIS3)COTYLEDON TRICHOME 1 (COT1), MYB5, ARPC5, MYB106, FZR2, HOMEODOMAIN GLABROUS 12(HDG12), BRANCHLESS TRICHOMES (BTL), HOMEODOMAIN GLABROUS 11 (HDG11), STICHEL (STI), IRREGULAR TRICHOME BRANCH1 (ITB1), KAKTUS (KAK), MYOSIN XI K, ATXIK, DYNAMIN-RELATED PROTEIN (DRP1A), ATRLCK VI_A3, ZWICHEL, FRC1, FRC3, FRC4, ITB2, ITB3, ITB4, RASTIFARI (RFI), STA, SUZ1, SUZ2, SUZ3, ZINC FINGER PROTEIN 5, ZINC FINGRE PROTEIN 6, ZINC FINGER PROTEIN 8, GL2-EXPRESSION MODULATOR (GEM), HARLEQUIN (HQL), TRANSPARENT TESTA 8 (TT8) in a plant of genus cannabis. ### Claim 26 (depends on 23) The method of claim 23, comprising modifying expression of a gene chosen from the R2R3-MYB, bHLH, WD40 repeat protein, or R3-MYB gene families. ### Claim 27 (depends on 11) The method of claim 11, comprising physically altering the DNA of the plant of genus cannabis.