A rotary cell culture device capable of rotating, at a predetermined speed, a culture vessel filled with a cell liquid obtained by suspending cells in a culture medium, the rotary cell culture device capable of performing culture of cells in a suspended state in a pseudo-microgravity environment, wherein
- the vessel comprises a front flange and a back flange which have a same circular outer shape and which are provided integrally with both ends of an outer cylinder part of the vessel,
- in a state where a head, of the vessel, serving as a port of a solution is closed by a detachable cap and a space, in the vessel, closed by a gasket of a plunger is filled with the cell liquid, each of lower portions of the front flange and the back flange is rotatably supported by a pair of rollers, and at least one of the rollers that are in contact with the front flange or the back flange is a driving roller which is rotated by a drive motor, and an other of the rollers that are in contact with the front flange or the back flange is a driven roller, and
- the rotary cell culture device comprises a restriction part for keeping an attitude by contacting and stopping an outer face extending in a radial direction of the front flange and the back flange.