Enzyme immobilization in hierarchical metal-organic frameworks
AssigneeNorthwestern University
InventorsOmar F. FARHA, Peng LI, Justin A. MODICA, Milan MRKSICH, Joseph T. HUPP
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Abstract
Enzyme-immobilizing MOFs and methods for their use in enzymatically catalyzed reactions are provided. The MOFs are channel-type MOFs that present a hierarchical pore structure comprising a first set of large channels sized for enzyme immobilization and a second set of smaller channels running alongside of the large channels that remain enzyme-free and allow for reactant delivery to the enzymes and product expulsion from the larger channels.
Claims20
An enzyme-immobilizing metal-organic framework compound comprising:
- a channel-type metal-organic framework compound having a hierarchical pore structure comprising a first set of large channels, a second set of small channels running alongside of the large channels, and openings defined between the large channels and the small channels, the large channels having a larger diameter than the small channels;
- and
- catalytically active enzymes immobilized in the large channels.
A method of enzymatically catalyzing a reaction using enzyme-immobilizing metal-organic framework compounds comprising:
- channel-type metal-organic framework compounds having a hierarchical pore structure comprising a first set of large channels, a second set of small channels running alongside of the large channels, and openings defined between the large channels and the small channels, the large channels having a larger diameter than the small channels;
- and
- catalytically active enzymes immobilized in the large channels of the channel-type metal-organic framework compounds,
- the method comprising:
- exposing the enzyme-immobilizing metal-organic framework compounds to a sample comprising chemical reactants and allowing the immobilied enzymes to catalyze a reaction between the reactants to form one or more reaction products.
Dependencies are inferred from the claim text.