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US 2019/0112330 A1Published application
Bile salt hydrolase probe and method of making and using the same
AssigneeBattelle Memorial Institute
InventorsAaron T. Wright, Kristoffer R. Brandvold, Susan Ramos-Hunter
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Abstract
Probe embodiments for targeting, identifying, and isolating enzymes exhibiting BSH activity as well as devices and kits that use the probes are described herein. Methods of making and using the probes, devices, and kits are also described. In some embodiments, probes, devices, and kits for targeting, identifying, and isolating enzymes in a biological sample are disclosed. In some embodiments, compositions and methods of treatment using the probes, devices, and kits disclosed herein are described.
Claims20
A probe having a structure satisfying Formula I
- wherein each of R1, R2, R3, and R4 independently is selected from hydrogen or —OR, wherein each R independently is selected from hydrogen;
- a counterion that balances a negative charge on the oxygen atom;
- aliphatic;
- heteroaliphatic;
- aromatic;
- or any combination of aliphatic, heteroaliphatic, or aromatic;
- R5 is hydrogen, aliphatic, heteroaliphatic, aromatic, or any combination of aliphatic, heteroaliphatic, or aromatic;
- the linker is aliphatic, heteroaliphatic, aromatic, or any combination of aliphatic, heteroaliphatic, or aromatic;
- DM is a detectable moiety;
- pDM is a detectable moiety precursor;
- PM is a photoactivatable moiety;
- AM is an anchor moiety;
- n is an integer ranging from 0 to 10;
- and m is an integer selected from 0 or 1.
11 more elements
A kit, comprising:
- a substrate;
- and
- a probe having a structure satisfying Formula I
- wherein each of R1, R2, R3, and R4 independently is selected from hydrogen or —OR, wherein each R independently is selected from hydrogen;
- a counterion that balances a negative charge on the oxygen atom;
- aliphatic;
- heteroaliphatic;
- aromatic;
- or any combination of aliphatic, heteroaliphatic, or aromatic;
- R5 is hydrogen, aliphatic, heteroaliphatic, aromatic, or any combination of aliphatic, heteroaliphatic, or aromatic;
- the linker is aliphatic, heteroaliphatic, aromatic, or any combination of aliphatic, heteroaliphatic, or aromatic;
- DM is a detectable moiety;
- pDM is a detectable moiety precursor;
- AM is an anchor moiety;
- n is an integer ranging from 0 to 10;
- and m is an integer selected from 0 or 1;
- and wherein the probe is covalently bound to a surface of the substrate via the AM group.
14 more elements
Dependencies are inferred from the claim text.