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US 2019/0102512 A1Published application
Method of analyzing microbiome
AssigneeYeda Research and Development Co. Ltd.
InventorsEran SEGAL, Eran ELINAV
Life of this patent
Estimating the term…
Abstract
A method of assessing the growth dynamics of a bacterium in a microbiome is disclosed.
Claims26
A method of determining the growth rate of a bacterium present in a microbiome comprising:
- (a) sequencing DNA fragments of a microbiome to obtain a plurality of nucleic acid sequencing data;
- (b) aligning said plurality of nucleic acid sequence data to at least one reference sequence, said reference sequence being of a genome of the bacterium;
- and
- (c) analyzing the frequency of at least one nucleotide positioned at the origin of replication of said genome and the frequency of at least one nucleotide positioned at the terminus of said genome, wherein the ratio of said frequencies is indicative of the growth rate of the bacterium.
1 more element
A method of determining the origin of replication of a replicating bacterium which is present in a mixed population of bacteria comprising:
- (a) sequencing DNA fragments of the genome of the bacterium to obtain a plurality of nucleic acid sequencing data;
- (b) aligning said plurality of nucleic acid sequence data to a reference sequence, said reference sequence being of the genome of the bacterium;
- and
- (c) analyzing the frequency of nucleotides across said genome of the bacterium;
- wherein the genomic location which corresponds to the highest number of reads is the origin of replication of the bacterium.
2 more elements
(canceled)
20-25. (canceled)
A method of diagnosing a disease of a subject comprising:
- (a) analyzing the growth dynamics of at least one bacterium in a microbiome sample of the subject;
- (b) comparing said growth rate of said at least one bacterium in said microbiome sample of the subject to the growth dynamics of said at least one bacterium in a microbiome of a subject having said disease, wherein when said growth dynamics of said at least one bacterium in said microbiome sample is statistically significantly similar to said growth dynamics of said at least one bacterium in said microbiome of said subject having said disease, it is indicative that the subject has said disease.
Dependencies are inferred from the claim text.