Two pass macro image

A digital slide scanning apparatus comprising:

• a stage configured to support a glass slide;
• a high-resolution camera;
• a first lens defining a macro image optical path to the high-resolution camera, wherein the first lens is configured to provide a field of view to the high-resolution camera, and wherein the field of view has a width that is substantially equal to a complete width of a glass slide;
• a first illumination system optically coupled with the first lens and configured for transmission-mode illumination of the field of view from below a glass slide on the stage;
• a second illumination system optically coupled with the first lens and configured for reflection-mode illumination of the field of view from above a glass slide on the stage, wherein the second illumination system is configured to angle illumination light in a pattern such that substantially none of its illumination light is reflected along the optical path to the high-resolution camera;
• and
• at least one processor configured to, while a stage is supporting a glass slide,
• while the field of view is illuminated by the first illumination system, capture a first macro image of a specimen on the glass slide using the high-resolution camera while the glass slide is under the first lens,
• while the field of view is illuminated by the second illumination system, capture a second macro image of the specimen on the glass slide using the high-resolution camera while the glass slide is under the first lens,
• identify image artifacts in the second macro image, and,
• based on the identified image artifacts in the second macro image, correct the first macro image to generate a modified first macro image.