US 2019/0101547 A1 · COMPOSITIONS AND METHODS FOR IDENTIFYING SUBJECTS AT RISK FOR TRAUMATIC BRAIN INJURY

Abstract

The present disclosure relates to compositions and methods for identifying a subject at risk for traumatic brain injury. In particular, the instant disclosure is directed to identification of the levels of the proteins MMP-9 (Matrix Metallopeptidase 9), NSE (Neuron Specific Enolase) and VCAM-1 (Vascular Cell Adhesion Molecule 1) in a subject sample, and correlation of these protein levels with the presence of intracranial injury. In certain embodiments, subject age, gender and hemoglobin level are also correlated with the presence of intracranial injury.

Claims36

Dependent claims

A kit for detecting expression levels of one or more protein markers selected from the group consisting of NSE, MMP-9, VCAM-1, protein markers of neuronal, glial, and/or axonal injury, protein markers of blood brain barrier dysfunction, vascular leakage, edema and/or wound repair neuroinflammation, protein markers of vascular disruption and/or cell adhesion, and protein markers of metabolic changes in a subject sample, wherein the kit comprises:

(i) one or more agents that are capable of specifically binding to said one or more protein markers;
(ii) reagents for detecting binding of the one or more agents to the one or more protein markers;
and
(iii) instructions for determining if the subject is at risk for traumatic brain injury, comprising:
(a) calculating a classification value for the subject based on a classification model of the one or more protein marker levels;
and
(b) identifying the subject as being at risk for traumatic brain injury when the classification value is greater than or less than a threshold value.

4 more elements

A method for identifying a subject at risk for traumatic brain injury comprising

(i) detecting expression levels of one or more protein markers selected from the group consisting of NSE, MMP-9, VCAM-1, protein markers of neuronal, glial, and/or axonal injury, protein markers of blood brain barrier dysfunction, vascular leakage, edema and/or wound repair neuroinflammation, protein markers of vascular disruption and/or cell adhesion, and protein markers of metabolic changes in a subject sample,
(ii) calculating a classification value for the subject based on a classification model of the one or more protein marker levels in the subject sample;
and
(iii) identifying the subject as being at risk for traumatic brain injury when the classification value is greater than or less than a threshold value.

1 more element

Dependencies are inferred from the claim text.

Compositions and methods for identifying subjects at risk for traumatic brain injury

Life of this patent

Compositions and methods for identifying subjects at risk for traumatic brain injury

Life of this patent

Abstract

Claims36