Antibody dispersion for measurement, production method thereof, kit for preparing antibody dispersion for measurement, and method for measuring biological substance
AssigneeKonica Minolta, Inc.
InventorsChie INUI, Akihiro MAEZAWA
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Abstract
An object of the invention is to provide an antibody dispersion for measurement, in which an antibody is favorably dispersed when used for a measurement, a production method thereof, a kit for preparing an antibody dispersion for measurement, and a measuring method of a biological substance. An antibody dispersion for measurement of the invention includes a monovalent metal salt and a divalent metal salt, in which the concentration of the monovalent metal salt is from 50 to 500 mM and the concentration of the divalent metal salt is from 1.0 to 50 mM.
Claims17
An antibody dispersion for measurement comprising a monovalent metal salt and a divalent metal salt,
- wherein the concentration of the monovalent metal salt is from 50 to 500 mM and the concentration of the divalent metal salt is from 1.0 to 50 mM.
A production method of an antibody dispersion for measurement comprising a monovalent metal salt and a divalent metal salt, wherein the concentration of the monovalent metal salt is from 50 to 500 mM and the concentration of the divalent metal salt is from 1.0 to 50 mM, and
- wherein the method comprises a step of adding a divalent metal salt to an antibody dispersion for preservation containing a monovalent metal salt at a concentration of from 10 to 300 mM.
A kit for preparing an antibody dispersion for measurement comprising an antibody dispersion for preservation containing a monovalent metal salt at a concentration of from 10 to 300 mM, and a solution containing a divalent metal salt,
- wherein an antibody contained in the antibody dispersion for preservation is a fluorescence-labeled antibody.
A measuring method of a biological substance for quantifying a target biological substance comprising the following steps (A) to (D):
- (A) a step for obtaining an antibody dispersion for measurement, in which a monovalent metal salt and a divalent metal salt are contained, and the concentration of the monovalent metal salt is from 50 to 500 mM and the concentration of the divalent metal salt is from 1.0 to 50 mM, by adding a divalent metal salt to an antibody dispersion for preservation, in which a fluorescence-labeled antibody and a monovalent metal salt are contained, and the concentration of the monovalent metal salt is from 10 to 300 mM,
- (B) a step for supplying a specimen containing a target biological substance onto a sensor chip,
- (C) a step for fluorescently labeling the specimen containing a target biological substance by supplying the antibody dispersion for measurement obtained in the step (A) onto the sensor chip having undergone the step (B), and
- (D) a step for detecting the fluorescence-labeled specimen containing a target biological substance by performing a fluorescence assay.
Dependencies are inferred from the claim text.