Assay for detecting hepatitis c virus (hcv)
AssigneeAbbott Molecular Inc.
InventorsDan Toolsie, Shihai Huang, Hong Su, Darren Drapp, William Caminiti, Kara Nordin, Sevim Arslan, Sara Jones
Life of this patent
Estimating the term…
Abstract
The disclosure is directed to methods, kits, and compositions, for amplifying and detecting a human hepatitis C virus (HCV) in a sample, which comprises a variety of combinations of forward oligonucleotide primers, reverse oligonucleotide primers, and oligonucleotide probes.
Claims19
A set of oligonucleotide sequences for amplifying and detecting a human hepatitis C virus (HCV) nucleic acid sequence in a sample, which comprises:
- (a) a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 1 or SEQ ID NO:
- 5;
- (b) a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 2, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, or SEQ ID NO:
- 9;
- (c) a first probe oligonucleotide sequence comprising SEQ ID NO:
- 3, SEQ ID NO:
- 10, SEQ ID NO:
- 11, or SEQ ID NO:
- 12;
- and
- (d) a second probe oligonucleotide sequence comprising SEQ ID NO:
- 4, SEQ ID NO:
- 13, or SEQ ID NO:
- 14, wherein each of the first and second probe oligonucleotide sequences comprises a detectable label.
A kit for detecting human hepatitis C virus (HCV) in a sample comprising
- (a) a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 1;
- (b) a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 2;
- (c) a first probe oligonucleotide sequence comprising SEQ ID NO:
- 3;
- (d) a second probe oligonucleotide sequence comprising SEQ ID NO:
- 4;
- (e) reagents for amplifying and detecting nucleic acid sequences;
- and
- (f) instructions for use,
- wherein each of the first and second probe oligonucleotide sequences comprises a detectable label.
A composition for amplifying and detecting a human hepatitis C virus (HCV) in a sample, which comprises:
- (a) a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 1 or SEQ ID NO:
- 5;
- (b) a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 2, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, or SEQ ID NO:
- 9;
- (c) a first probe oligonucleotide sequence comprising SEQ ID NO:
- 3, SEQ ID NO:
- 10, SEQ ID NO:
- 11, or SEQ ID NO:
- 12;
- and
- (d) a second probe oligonucleotide sequence comprising SEQ ID NO:
- 4, SEQ ID NO:
- 13, or SEQ ID NO:
- 14, wherein each of the first and second probe oligonucleotide sequences comprises a detectable label.
Dependencies are inferred from the claim text.