Assay for detecting human immunodeficiency virus (hiv)
AssigneeAbbott Molecular Inc.
InventorsJeffrey Wuitschick, Shihai Huang, Tomasz Krupinski, John Karavitis, John Salituro, Anna Sobol
Life of this patent
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Abstract
The disclosure is directed to methods, kits, and compositions for amplifying and detecting a human immunodeficiency virus-1 (HIV-1) in a sample, which comprises a variety of combinations of forward oligonucleotide primers, reverse oligonucleotide primers, and oligonucleotide probes.
Claims15
A set of oligonucleotide sequences for amplifying and detecting one or more human immunodeficiency virus-1 (HIV-1) nucleic acid sequences in a sample, which comprises:
- (a) a primer and probe set that amplifies and detects at least a portion of the HIV-1 integrase (INT) gene comprising a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 1, a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 2, and a probe oligonucleotide sequence, and
- (b) a primer and probe set that amplifies and detects at least a portion of an HIV-1 long terminal repeat (LTR) region, which comprises a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 5, a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 6, a first probe oligonucleotide sequence, and a second probe oligonucleotide sequence,
- wherein each of the probe oligonucleotide sequences comprises a detectable label and/or a quencher moiety.
A kit for detecting human immunodeficiency virus-1 (HIV-1) in a sample comprising:
- (a) a primer and probe set that amplifies and detects at least a portion of the HIV-1 integrase (INT) gene comprising a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 1, a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 2, and a probe oligonucleotide sequence, and
- (b) a primer and probe set that amplifies and detects at least a portion of the HIV-1 long terminal repeat (LTR) region, which comprises a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 5, a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 6, a first probe oligonucleotide sequence, and a second probe oligonucleotide sequence,
- (c) reagents for amplifying and detecting nucleic acid sequences;
- and
- (d) instructions for use, wherein each of the probe oligonucleotide sequences comprises a detectable label and/or a quencher moiety.
A composition for amplifying and detecting human immunodeficiency virus-1 (HIV-1) in a sample, which comprises:
- (a) a primer and probe set that amplifies and detects at least a portion of the HIV-1 integrase (INT) gene comprising a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 1, a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 2, and a probe oligonucleotide sequence, and
- (b) a primer and probe set that amplifies and detects at least a portion of the HIV-1 long terminal repeat (LTR) region, which comprises a forward primer oligonucleotide sequence comprising SEQ ID NO:
- 5, a reverse primer oligonucleotide sequence comprising SEQ ID NO:
- 6, a first probe oligonucleotide sequence, and a second probe oligonucleotide sequence, wherein each of the probe oligonucleotide sequences comprises a detectable label and/or a quencher moiety.
Dependencies are inferred from the claim text.