US 2019/0100811 A1 · PHAGE-BASED DETECTION METHOD FOR ANTIMICROBIAL SUSCEPTIBILITY TESTING AND IDENTIFICATION OF BACTERIAL SPECIES

Abstract

Methods for determining bacterial identity and susceptibility or resistance to antibiotic or antimicrobial agents are provided. In one embodiment, the bacteria is cultured in the presence or absence or the antibiotic agent to generate a plurality of primary cultures, which are then cultured in the presence or absence of transforming phages to generate a first secondary culture that comprise transformed bacteria that have been treated with the antibiotic agent and a second secondary culture that comprises transformed bacteria that have not been treated with the antibiotic agent. The recombinant phages are specific to the bacteria and comprise a heterologous marker. The susceptibility or resistance of the bacteria to the antibiotic or antimicrobial agent is determined by comparing a level or activity of the marker in the first and second secondary cultures.

Claims23

Dependent claims

A method for simultaneously identifying a bacterial species and determining a susceptibility of the bacterial species to a test antimicrobial agent, comprising,

(a) providing a transforming phage specific to the bacterial species, wherein the transforming phage is an engineered or recombinant phage having a gene for encoding a marker, the gene being heterologous to both humans and the bacterial species;
(b) preparing a first culture by culturing the bacterial species in the absence of the test antimicrobial agent to generate a primary test antimicrobial-free culture and culturing the primary test antimicrobial-free culture in the presence of the transforming phage;
(c) preparing a second culture by culturing the bacterial species in the presence of the test antimicrobial agent to generate a primary test antimicrobial-containing culture;
culturing the primary test antimicrobial-containing culture in the presence of the transforming phage;
and
(d) analyzing the first and second cultures to determine a level or activity of the marker in each of the first and second cultures.

3 more elements

Dependencies are inferred from the claim text.

Phage-based detection method for antimicrobial susceptibility testing and identification of bacterial species

Life of this patent

Phage-based detection method for antimicrobial susceptibility testing and identification of bacterial species

Life of this patent

Abstract

Claims23