Compositions and methods for detecting mutations in jak2 nucleic acid

A method for detecting a mutated JAK2 nucleic acid in a sample obtained from a human, comprising:

• (a) contacting the sample with a detectably labeled nucleic acid probe that specifically hybridizes to a mutant JAK2 nucleic acid but not to a wild-type JAK2 nucleic acid and comprises the mutation, wherein the mutation is selected from among group consisting of:
• an adenine to thymine mutation at a nucleotide position corresponding to nucleotide position 2091 of SEQ ID NO:
• 1 in the JAK2 nucleic acid;
• a thymine to cytosine mutation at a nucleotide position corresponding to nucleotide position 2127 of SEQ ID NO:
• 1 in the JAK2 nucleic acid;
• a thymine to cytosine mutation at a nucleotide position corresponding to nucleotide position 2133 of SEQ ID NO:
• 1 in the JAK2 nucleic acid;
• a thymine to guanine mutation at a nucleotide position corresponding to nucleotide position 2160 of SEQ ID NO:
• 1 in the JAK2 nucleic acid;
• a cytosine to thymine mutation at a nucleotide position corresponding to nucleotide position 2180 of SEQ ID NO:
• 1 in the JAK2 nucleic acid;
• a cytosine to thymine mutation at a nucleotide position corresponding to nucleotide position 2204 of SEQ ID NO:
• 1 in the JAK2 nucleic acid;
• and
• an adenine to guanine mutation at a nucleotide position corresponding to nucleotide position 2204 of SEQ ID NO:
• 1 in the JAK2 nucleic acid;
• and
• (b) detecting the mutant JAK2 nucleic acid when a hybrid is formed between the detectably labeled nucleic acid probe and the mutant JAK2 nucleic acid.