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US 2019/0100800 A1Published application
Epigenetic Method for the Identification of Follicular T-Helper-(TFH-) Cells
AssigneeEpiontis GmbH
InventorsSven OLEK, Ulrich HOFFMÜLLER
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AssigneeEpiontis GmbH
InventorsSven OLEK, Ulrich HOFFMÜLLER
Estimating the term…
The present invention relates to a method, in particular an in vitro method, for identifying follicular helper T cells, comprising analyzing the methylation status of at least one CpG position in the mammalian gene region for leukemia inhibitory factor (LIF), wherein a demethylation of said gene region is indicative for a follicular helper T cell, when compared to a non-follicular helper T cell. The analyses according to the invention can identify follicular helper T cells on an epigenetic level and distinguish them from all other cells in complex samples, such as, for example, other blood cells. The present invention furthermore provides an improved method for quantifying follicular helper T cells in complex samples. The method can be performed without a step of purifying and/or enriching cells, preferably in whole blood and/or non-trypsinized tissue.
A method for identifying follicular helper T cells in a sample, comprising analyzing the methylation status of at least one CpG position in a mammalian gene region for leukemia inhibitory factor (LIF), wherein said gene region as analyzed is positioned based on/according to SEQ ID NO:
A kit for identifying, quantifying, and/or monitoring follicular helper T cells in a mammal based on an analysis of the bisulfite accessibility of CpG positions in the gene region of LIF, comprising a) a bisulfite reagent, and b) materials for the analysis of the methylation status of CpG positions selected from the CpG positions in the region according to SEQ ID NO:
An oligomer according to any of SEQ ID NOs:
Dependencies are inferred from the claim text.