Back to results
US 2019/0100787 A1Published application
Compositions and methods for detection of trichomonas vaginalis
AssigneeRoche Molecular Systems, Inc.
InventorJody Harris
Life of this patent
Estimating the term…
Abstract
Methods for the rapid detection of the presence or absence of Trichomonas vaginalis (TV) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the TV beta tubulin gene, along with kits are provided that are designed for the detection of TV.
Claims11
A method of detecting Trichomonas vaginalis (TV) in a sample, the method comprising:
- performing an amplifying step comprising contacting the sample with a set of TV beta tubulin gene primers to produce an amplification product if TV beta tubulin nucleic acid is present in the sample;
- performing a hybridizing step comprising contacting the amplification product with one or more detectable TV beta tubulin gene probes;
- and
- detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of TV in the sample and wherein the absence of the amplification product is indicative of the absence of TV in the sample;
- wherein the set of TV beta tubulin gene primers comprise a first primer comprising a first oligonucleotide sequence selected from the group consisting of SEQ ID NOs:
- 1-2, or a complement thereof, and a second primer comprising a second oligonucleotide sequence selected from the group consisting of SEQ ID NOs:4-6, or a complement thereof;
- and
- wherein the one or more detectable TV beta tubulin gene probes comprises a third oligonucleotide sequence selected from the group consisting of SEQ ID NOs:
- 8-12, or the complement thereof.
6 more elements
A kit for detecting a nucleic acid of Trichomonas vaginalis (TV) comprising:
- a first primer comprising a first oligonucleotide sequence selected from the group consisting of SEQ ID NOs:
- 1-2, or a complement thereof;
- a second primer comprising a second oligonucleotide sequence selected from the group consisting of SEQ ID NOs:
- 4-6, or a complement thereof;
- and
- a fluorescently detectably labeled probe comprising a third oligonucleotide sequence selected from the group consisting of SEQ ID NOs:
- 8-12, or a complement thereof, the detectably labeled probe configured to hybridize to an amplicon generated by the first primer and the second primer.
4 more elements
Dependencies are inferred from the claim text.