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AssigneeCorbion Biotech, Inc.
InventorsScott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walter Rakitsky, Xinhua Zhao, Riyaz Bhat
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Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
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A method of producing a triglyceride oil, the method comprising:
A recombinant oleaginous microbial cell, the recombinant microbial cell comprising an exogenous gene that encodes a β-ketoacyl synthase II (KASII) peptide, wherein fatty acids of triglyceride oil produced by the recombinant microbial cell is enriched in C18 fatty acids or reduced in C16 fatty acids.
Dependencies are inferred from the claim text.