Design method for synthetic genes
AssigneeJnc Corporation
InventorSatoshi Inouye
Life of this patent
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Abstract
The present invention provides a method of designing an optimized gene which comprises altering a nucleotide sequence of a target protein gene, so that only preferential codons with high frequency of use in human cells are selected and a GC content of not less than 60% is achieved. A gene design method which involves the feature “only preferential codons with high frequency of use are selected and a GC content of not less than 60% is achieved” can be established as a general rule for preparing proteins with high expression level, in order to obtain chemically synthesized genes for proteins capable of high-level expression in eukaryotes.
Claims12
1-40. (canceled)
An isolated polynucleotide comprising the sequence of SEQ ID NO:
- 16.
A kit comprising:
- a) at least one of (i) an isolated polynucleotide comprising the sequence of SEQ ID NO:
- 16, (ii) a recombinant expression vector comprising a polynucleotide comprising the sequence of SEQ ID NO:
- 16 under the control of a promoter capable of functioning in a mammalian cell, and (iii) an isolated recombinant mammalian cell comprising a polynucleotide comprising the sequence of SEQ ID NO:
- 16, and
- b) luciferin and/or a luciferin analogue.
Dependencies are inferred from the claim text.