US 2019/0100768 A1Published application

Tospovirus Resistant Plants and Methods Thereof

OwnerParthasarathi Bhattacharya, Suresh Atluri +2inventors

InventorsParthasarathi Bhattacharya, Suresh Atluri, Surinder Kumar Tikoo, Charles Pick

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Abstract

The present invention provides tomato plants exhibiting resistance to TOSPO (GBNV) Virus exhibiting the traits of healthy and evenly ripened fruits without brown spots, reduced wilting of the top portion of the tomato plants and reduced dark spots on the leaves and stems of the tomato plant, and materials useful for improving tomato yield. In particular, the present invention provides a path to development of hybrid tomato plants exhibiting resistance to TOSPO virus infection, seed of hybrid tomato plants exhibiting TOSPO virus resistance, and methods of producing the same. Methods described herein involve the use of marker assisted selection and marker assisted backcrossing utilizing molecular markers associated with a tomato TOSPO Resistance phenotype.

Claims19

Dependent claims

A method for producing a tomato plant, wherein the tomato plant exhibits TOSPO Virus resistance, comprising:

(a) providing a donor tomato plant, wherein the donor plant exhibits TOSPO virus resistance;
(b) transferring at least one nucleic acid from the first tomato plant to one or more tomato plants, wherein the recipient tomato plants do not exhibit TOSPO virus resistance, and wherein the transfer results in the introduction of genomic material from the donor tomato plant in a corresponding region of one or more recipient tomato plants;
and
(c) identifying and selecting, from the one or more recipient tomato plants, a tomato plant that comprises within its genome at least one quantitative trait locus (QTL) for TOSPO virus resistance, wherein the QTL is selected from the group consisting of:
QTL1, QTL2 and TOSPO viral resistance related traits thereof, and wherein QTL1 is indicated by a genomic region on chromosome 1 and QTL2 is indicated by a genomic region on chromosome 2.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application: 15/555,937Filed 28 Aug 2017
Published: 4 Apr 2019
Term ends: Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12N 15/8283
For biotic stress resistance, pathogen resistance, disease resistance: for virus resistance
A01H 1/021
Methods or apparatus for hybridisation; Artificial pollination ; Fertility: methods of breeding using interspecific crosses, i.e. interspecies crosses
A01H 1/045
Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection: using molecular markers
A01H 5/08
Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy: fruits