Method for increasing nitrogen-use efficiency in plants
OwnerXiaorong Fan, Lin Caoinventors
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Abstract
Disclosed herein is a method of improving yield, growth and/or nitrogen use efficiency in plants comprising altering the expression profile of NRT. Also provided methods of making such plants, including nucleic acid constructs and genetically altered plants with the above traits.
Claims52
A method for increasing growth, yield, biomass, agricultural nitrogen use efficiency (ANUE), N recovery efficiency (NRE), stress tolerance and/or total N content of a plant and/or mitigating the effects of stress on a plant, the method comprising altering the expression profile of a NRT2 nucleic acid in a plant, wherein preferably the NRT2 nucleic acid comprises a sequence encoding a NRT2.1, NRT2.2 and/or NRT2.3a polypeptide as defined in SEQ ID NOs:
- 2, 4 and 6 respectively, or a functional homologue or variant thereof.
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A nucleic acid construct comprising a nucleic acid sequence encoding any one of SEQ ID Nos:
- 2, 4 or 6, a functional variant or homolog thereof, operably linked to a regulatory sequence, wherein said regulatory sequence is a nitrate-inducible promoter, and wherein preferably the nitrate-inducible promoter is a NAR2.1 promoter comprising a sequence as defined in SEQ ID No:
- 7 or a functional homologue or variant thereof.
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A method of producing a mutant plant that has increased growth, biomass, yield, agricultural nitrogen use efficiency (ANUE), N recovery efficiency (NRE), improved stress tolerance and/or total N content of a plant or of mitigating the effects of stress on a plant, the method comprising introducing a mutation into the plant genome, wherein said mutation is introduced by mutagenesis or targeted genome editing, and wherein said mutation introduces at least one or more additional copy of
- a NRT2.1, 2.2 or 2.3a gene sequence such that at least one sequence is operably linked to an endogenous nitrate-inducible promoter, preferably an endogenous NAR2.1 promoter sequence;
- a NAR 2.1 promoter sequence, such that said promoter sequence is operably linked to at least one endogenous NRT2.1, 2.2 or 2.3a gene sequence and/or
- a NRT 2.1, NRT 2.2 or NRT 2.3a gene sequence operably linked to a NAR2.1 promoter sequence.
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The genetically altered plant of claim 45, wherein said plant carries a mutation in its genome and wherein said mutation introduces one or more additional copy of a
- a NRT2.1, 2.2 or 2.3a gene sequence such that at least one sequence is operably linked to an endogenous nitrate-inducible promoter, preferably an endogenous NAR2.1 promoter sequence;
- a NAR 2.1 promoter sequence, such that said promoter sequence is operably linked to at least one endogenous NRT2.1, 2.2 or 2.3a gene sequence and/or
- a NRT 2.1, NRT 2.2 or NRT 2.3a gene sequence operably linked to a NAR2.1 promoter sequence;
- into the plant genome.
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A genetically altered plant characterised by a lower expression ratio of NRT2.1:NAR2.1, NRT2.2:
- NAR 2.1 and/or NRT2.3a:NAR2.1 ratio compared to said ratio in a control plant.
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Dependencies are inferred from the claim text.