Ap2 domain transcription factor odp2 (ovule development protein 2) and methods of use
AssigneePioneer Hi-Bred International, Inc.
InventorsWILLIAM J. GORDON-KAMM, TIMOTHY G. HELENTJARIS, KEITH S. LOWE, BO SHEN, MITCHELL C. TARCZYNSKI, PEIZHONG ZHENG
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Abstract
Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
Claims22
1-3. (canceled)
A plant comprising a heterologous polynucleotide operably linked to a promoter that drives expression in the plant, wherein said polynucleotide is selected from the group consisting of:
- (a) the polynucleotide comprising SEQ ID NO:1, 3, 25 or 27;
- (b) the polynucleotide encoding the amino acid sequence of SEQ ID NO:2, 26 or 28;
- (c) the polynucleotide having at least 80% sequence identity to SEQ ID NO:1, 3, 25 or 27, wherein said polynucleotide encodes a polypeptide having ODP2 activity;
- (d) the polynucleotide having at least 450 consecutive nucleotides of SEQ ID NO:1, 3, 25, 27, or a complement thereof;
- and,
- (e) the polynucleotide encoding an amino acid sequence having at least 80% sequence identity to SEQ ID NO:2, 26 or 28, wherein said polynucleotide encodes a polypeptide having ODP2 activity.
5-25. (canceled)
A polynucleotide designed for expression in a plant selected from the group consisting of:
- (a) the polynucleotide encoding the amino acid sequence of SEQ ID NO:23;
- (b) the polynucleotide encoding an amino acid sequence having at least 85% sequence identity to SEQ ID NO:23, wherein said polynucleotide encodes a polypeptide comprising two AP2 domains and that renders a plant cell embryogenic when expressed in the plant cell;
- and,
- (c) the polynucleotide comprising a full length complement of the polynucleotide of (a)-(b);
- wherein the polynucleotide is operably linked to a heterologous regulatory sequence.
A plant comprising a heterologous polynucleotide operably linked to a promoter that drives expression in the plant, wherein said polynucleotide is selected from the group consisting of:
- (a) the polynucleotide encoding the amino acid sequence of SEQ ID NO:23;
- (b) the polynucleotide encoding an amino acid sequence having at least 85% sequence identity to SEQ ID NO:23, wherein said polynucleotide encodes a polypeptide comprising two AP2 domains and that renders a plant cell embryogenic when expressed in the plant cell;
- and,
- (c) the polynucleotide comprising a full length complement of the polynucleotide of (a)-(b).
A method of increasing the activity of a polypeptide in a plant comprising introducing into said plant a polynucleotide selected from the group consisting of:
- (a) the polynucleotide encoding the amino acid sequence of SEQ ID NO:23;
- and,
- (b) the polynucleotide encoding an amino acid sequence having at least 85% sequence identity to SEQ ID NO:23, wherein said polynucleotide encodes a polypeptide comprising two AP2 domains and that renders a plant cell embryogenic when expressed in the plant cell, wherein the polynucleotide is operably linked to a heterologous regulatory sequence.
Dependencies are inferred from the claim text.