Compositions and methods for enhanced gene expression and viral replication
AssigneeDuke University
InventorsBryan R. Cullen, Edward M. Kennedy, Hal P. Bogerd, Anand Kornepati, Nicholas Heaton, David Courtney
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Abstract
The invention generally relates to compositions (including polynucleotides, constructs, fusion proteins, vectors, and cells) and methods of using such compositions for enhancing gene expression, protein production and viral replication. More specifically, the invention relates to use of m6A sequences and/or YTHDF polypeptides to enhance gene expression or viral replication.
Claims37
A construct comprising a promoter operably connected to a polynucleotide encoding a polypeptide, wherein the polynucleotide comprises at least two engineered m6A sequences.
A construct comprising in the 5′ to 3′ direction of at least one strand of the construct, a heterologous coding sequence encoding a heterologous polypeptide, and a UTR sequence, wherein the UTR sequence comprises at least two m6A sequences.
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A construct comprising a promoter operably connected to a polynucleotide comprising an insert site or encoding a heterologous polypeptide, and at least one m6A sequence.
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A construct comprising (i) a heterologous coding sequence encoding a heterologous polypeptide, and (ii) a UTR sequence, the UTR sequence comprising at least one RNA-binding polypeptide recognition sequence.
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A cell engineered to overexpress a YTHDF polypeptide.
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Dependencies are inferred from the claim text.